Engineering a Highly Adhesive, Antimicrobial and Osteoinductive Composite Hydrogels for Treatment of Pre-Implant Diseases

Poster Session: 

B

Presenter: 

Zheng Cao

Authors: 

Ehsan Shirzaei Sani1, Zheng Cao1, Roberto Portillo Lara1,2, Zahra Aldawood3, Giuseppe Intini3,4, Nasim Annabi1,5*

Author Affiliation: 

"1Department of Chemical Engineering, Northeastern University, Boston, MA, USA. 3Centro de Biotecnología FEMSA, Tecnológico de Monterrey, Monterrey, NL, México. 2Division of Periodontology, Department of Oral Medicine, Infection, and Immunity, Harvard School of Dental Medicine, Boston, MA, USA. 4Harvard Stem Cell Institute, Cambridge, MA, USA. 5Biomaterials Innovation Research Center, Brigham and Women’s Hospital, Harvard Medical School, Boston, MA, USA."

Abstract: 

As dental implants have become the standard of care for tooth replacement, the number of patients affected by pre-implant diseases (PIDs) is increasing. These diseases are characterized by the inflammation of the soft tissue surrounding the implants, infection and bone loss around the implants. Since implant placements continue increasing, it is predicted that PIDs will become one of the most significant dental diseases of the future. In the current study, we aimed to engineer novel antimicrobial and osteoinductive hydrogels for treatment of PIDs. The engineered hydrogels are composed of gelatin methacryloyl (GelMA), osteoinductive silicate nanoparticles (SN) and antimicrobial peptide (AMP). The composite hydrogels could be rapidly crosslinked in situ using LED dental curing light and exhibited high in vitro cytocompatibility, osteogenic differentiation as well as remarkable antimicrobial activity against Porphyromonas gingivalis, a pathogenic bacterium found in PIDs. Furthermore, our in vivo studies showed that the engineered hydrogels could effectively seal calvarial bone defects in mice for up to 42 days and induce bone regeneration. Overall, our results suggest that the engineered antimicrobial and osteoinductive hydrogels can be used an effective strategy for treatment of PIDs.