Instant Hydrogelation Inspired by Inflammasomes

Presenter: 

Huaimin Wang

Authors: 

Huaimin Wang, Zhaoqianqi Feng, Alvin Lu, Yujie Jiang, Hao Wu, and Bing Xu *

Author Affiliation: 

Dr. H. Wang, Ms. Z. Feng, Ms. Y. Jiang, and Prof. Dr. B. Xu Department of chemistry Brandeis University Dr. A. Lu, and Prof. H. Wu Department of Biological Chemistry and Molecular Pharmacology Harvard Medical School

Abstract: 

Based on the recent near-atomic structures of the PYRIN domain of ASC in the protein filament of inflammasomes and the observation of the active form of vitamin B6 (pyridoxal phosphate, P5P) modulates the self-assembly of ASC, we rationally designed an N-terminal capped nonapeptide (Nap-FFKKFKLKL, 1) to form supramolecular nanofibers consisting of alpha-helix. The addition of P5P to the solution of 1 results in a hydrogel almost instantly (about 4 seconds). Several other endogenous bioactive small molecules (e.g., pyridoxal, folinic acid, ATP, and AMP) also convert the solution of 1 to a hydrogel. As the demonstration of correlating supramolecular assemblies of peptides and the relevant protein epitopes, this work illustrates a bioinspired approach to develop supramolecular structures modulated by endogenous bioactive small molecules.