In this work, bisphenol A regioselective oxidation using tyrosinase was investigated in toluene solution with oxygen and little water. Due to the low activity of tyrosinase in organic solution, mesoporous carbon (MPC) coated with Polyethylenimine (PEI), which had a large surface area, large pore volume, and strong affinity with tyrosinase, was used as a platform for enzyme immobilization. Compared with MPC, polyethylene glycol (PEG)-coated MPC and polyacrylate (PAL)-coated MPC, PEI-coated MPC was the best immobilization platform for tyrosinase. The rate of oxidation p-cresol by tyrosinase/MPC/PEI, tyrosinase/MPC/PAL, tyrosinase/MPC/PEG and tyrosinase/MPC for p-cresol were 0.0081, 0.004, 0 and 0, respectively. After optimizing the experimental conditions, the rate of oxidation bisphenol a by tyrosinase/MPC/PEI in toluene with 6% water was 0.039. The stable product of bisphenol A could be used as a potential monomer for polymer or metal-organic framework. Our reaction system avoided adsorbing the substrate and product onto the surface of the carbon material and assessed the enzyme activity after immobilization onto carbon material, which might ignite the new engine for carbon material as enzyme immobilization platform.